Expression vector map. Regions encoding epitope tags.

Expression vector map. Basic Vector Information The pcDNA3. It shows low cytotoxicity and high genetic stability. This is due to the high selectivity of the pET system’s bacteriophage T7 RNA polymerase for its cognate promoter sequences, the high level of activity of the polymerase and the high translation Basic Vector Information pET-28a (+) is a bacterial expression vector designed for the production of N-terminally 6xHis-tagged proteins with a thrombin cleavage site. 1. Basic Vector Information The pCAGGS vector has several distinctive features. This vector is particularly suitable for proteins that require post-translational processing to remove the His-tag. The pET expression system1 is one of the most widely used systems for the cloning and in vivo expression of recombinant proteins in E. For other reading frames, use pET-28b(+) or pET-28c(+). See Appendix 2 (Control regions for pGEX vectors) for the control regions of the 13 vectors. Once the expression vector is inside the cell, the protein that is encoded by the gene is produced by the cellular-transcription and translation machinery ribosomal complexes. bgzwqhy p57 nvy7cf oi98w gquxtd toylw kzuxs nnqmvr ot tcg